Dissolved Oxygen and BOD Meter Research Grade



Item no. HI-5421-02

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1 185 €

The HI5421 is an advanced research grade benchtop Dissolved Oxygen and BOD meter that is completely customizable a large color LCD, capacitive

touch keys, and USB port for computer connectivity. The HI5421 is rich in features including data logging, alarm limits, comprehensive GLP, and many more while retaining simplicity in use with both dedicated key for routine operation and virtual keys that guide the user through setup options.

Capacitive touch keypad

Built in Barometer for automatic pressure compensation

BOD, OUR and SOUR methods


The HI5421 is supplied with the HI76483 Clark-Type Polarographic probe that measures a wide range of dissolved oxygen from0.0 to 600% saturation and 0.00 to 90.00 mg/L (ppm). The HI76483 is only 12 mm in diameters has a built in thermistor temperature sensor that compensates for temperature variations from 0 to 50 oC.

The HI5421 can be calibrated automatically to two points (0% and 100% saturation or 0.00 mg/L and 8.26 mg/L) or to a user-defined value. The calibration data including date, time, and calibration points can be accessed at any time along with the current measurement by selecting the Good Laboratory Practice (GLP) display option.

Readings are compensated for barometric pressure by a built in pressure transducer located in the meter. Calibration of the barometric pressure is single point with manual entry of current value obtained from local weather service or other device. Barometric pressure is displayed in a multiple choice of units including mmHg, mbar, kPa, mHg, psi, and atm.  The HI5421 also allow for automatic salinity compensation with a selectable salinity range of 0 to 45 g/L.

The HI5421 offers three additional measurement modes: Biological Oxygen Demand (BOD), Oxygen Uptake Rate (OUR) and Specific Oxygen Uptake Rate (SOUR). Simply enter values and take readings at appropriate times and the meter will automatically calculate the values. 

Three selectable logging modes are available: automatic, manual and AutoHold logging. Up to 100,000 data points can be recorded in 100 lots with 50,000 records max/lot and exported to a computer for data review and storage.

Features at-a-glance

Highly Customizable User Interface – The user interface of the HI5421 allows the user to show measurements in various modes: basic measurement with or without GLP information, real-time graphing, and logging data. Calibration stability criteria can be adjusted from fast, moderate, and accurate. Programmable alarm limits can be set to inside or outside allowable limits.

Color Graphic LCD – The HI5421 features a color graphic LCD with on-screen help, graphic, and custom color configurations. The display allows for real-time graphing and the use of virtual keys provide for an intuitive user interface.

Capacitive Touch – The HI5421 features sensitive capacitive touch buttons for accurate keystrokes when navigating menus and screens. There are four dedicated keys that are used for routine operations including calibration and switching measurement modes and four virtual keys that change based upon use. The capacitive touch technology ensures the buttons never get clogged with sample residue. 

Built in Barometer – The HI5421 has a built in pressure transducer to automatic barometric pressure compensation of all dissolved oxygen measurements. The barometric pressure can be calibrated to a single point. 

BOD, OUR and Sour Measurement Modes – An additional three measurement modes are available to measure Biological Oxygen Demand (BOD), Oxygen Uptake Rate (OUR) and Specific Oxygen Uptake Rate (SOUR). Simply enter values and take readings at appropriate times and the meter will automatically calculate the values.

Choice of Calibration – Automatic standard recognition is available for two points at 0% and 100% saturation or 0 mg/L and 8.26 mg/L.  A user standard option is available for a user defined value.

GLP Data – HI5421 includes a GLP Feature thatallows users to view calibration data and calibration expiration information at the touch of a key. Calibration data include date, time, and calibration points.

Data Logging – Three selectable logging modes are available on the HI5421: automatic, manual, and AutoHold logging. Automatic and manual logs up to 100 lots with 50,000 records max/lot, with up to 100,000 total data points. Automatic logging features the option to save data according to sampling period and interval.

Data Transfer – Data can be transferred to a PC with the included USB cable and HI92000 software. 

Contextual Help – Contextual help is always available through a dedicated “HELP” key. Clear tutorial messages and directions are available on-screen to quickly and easily guide users through setup and calibration.  The help information displayed is relative to the setting/option being viewed.

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Ordering Information: HI5421 is supplied with HI76483 DO probe, HI76404W electrode holder, HI7041S electrolyte solution (30 mL), HI764080A DO membrane caps (2), 12 VDC adapter, instrument quality certificate and instruction manual

Warranty: 2 year limited warranty (meter), 6 month limited warranty (sensor)

Connectivity USB
Dimensions 160 x 231 x 94 mm (6.3 x 9.1 x 3.7”)
Display color graphic LCD with on-screen help, graphing, and custom color configuration
DO Accuracy ±1.5% of reading ±1 digit
DO Calibration automatic using single or two-point calibration; user calibration single point
DO Range 0.00 to 90.00 ppm (mg/L); 0.0 to 600.0 % saturation
DO Resolution 0.01 ppm (mg/L); 0.1% saturation
DO Salinity Compensation 0 to 45 ppt
DO Temperature Compensation 0.0 to 50.0 °C; 32.0 to 122.0 °F; 237.1 to 323.1 K
Electrode/Probe HI76483 thin body, polarographic dissolved oxygen probe with internal temperature sensor and 1 m (3.3’) cable (included)
Environment 0 to 50°C (32 to 122°F; 273 to 323 K), RH max 95% non-condensing
GLP calibration data including date, time, and calibration points
Logging record: 100,000 data point storage, 100 lots with 50,000 records/lot; interval: settable between 1 second and 180 minutes max log time; type: automatic, manual, AutoHold
Measurement Modes direct DO; BOD (biochemical oxygen demand); OUR (oxygen uptake rate); SOUR (specific oxygen uptake rate)
Notes **Reduced to actual probe limits
Power Supply 12 Vdc (power adapter included)
Temperature Accuracy ±0.2 °C; ±0.4 °F; ±0.2 K (without probe)
Temperature Range -20.0 to 120.0 ºC**; -4.0 to 248.0 ºF**; 253.15 to 393.15 K**
Temperature Resolution 0.1 °C, 0.1 °F, 0.1 K
Weight 1.2 kg (2.64 lbs.)
Barometric Pressure Range 450 to 850 mmHg; 560 to 1133 mBar (additional units available (kPa, mHg, psi, atm))
Barometric Pressure Resolution 1 mm Hg
Barometric Pressure Accuracy ±3 mm Hg + 1 least significant digit

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